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Food
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BAM Media M59: Heart infusion agar
January 2001
Bacteriological Analytical Manual
M59
Heart infusion agar (HIA)
(Difco)
| Beef heart infusion (Difco) | 500 g |
| Tryptose | 10 g |
| NaCl | 5 g |
| Agar | 15 g |
| Distilled water | 1 liter |
Or
| Blood Agar Base No. 2 (Difco) | 1 liter |
| Proteose peptone | 15 g |
| Liver digest | 2.5 g |
| Yeast extract | 5 g |
| NaCl | 5 g |
| Agar | 12 g |
Autoclave either base at 121°C for 15 min. If preparing blood agar, reduce water to 950 ml. Add 50 ml defibrinated (whole or lysed) horse blood and FBP (4 ml to agar + blood) after autoclaving and cooling to 48°C. Final pH, 7.4 ± 0.2.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.
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