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Food
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BAM Media M138: Simmons Citrate Agar
January 2001
Bacteriological Analytical Manual
M138
Simmons Citrate Agar
| Sodium citrate* | 2 g |
| NaCl | 5 g |
| K2HPO4 | 1 g |
| NH4H2PO4 | 1 g |
| MgSO4 | 0.2 g |
| Bromthymol blue | 0.08 g |
| Agar | 15 g |
| Distilled water | 1 liter |
Heat gently with occasional agitation. Boil 1-2 min until agar dissolves. Fill 13 x 100 or 16 x 150 mm screw-cap tubes 1/3 full. Autoclave 15 min at 121°C. Before medium solidifies, incline tubes to obtain 4-5 cm slants and 2-3 cm butts. Final pH, 6.8 ± 0.2.
*Difco does not specify waters of hydration.
Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.
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