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Food
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BAM Media M136: Shigella Broth
January 2001
Bacteriological Analytical Manual
M136
Shigella Broth
Base
| Tryptone | 20 g |
| K2HPO4 | 2 g |
| KH2PO4 | 2 g |
| NaCl | 5 g |
| Glucose | 1 g |
| Tween 80 | 1.5 ml |
| Distilled water | 1 liter |
Autoclave 15 min at 121°C. Final pH, 7.0 ± 0.2.
Novobiocin solution
Weigh 50 mg novobiocin into 1 liter distilled water.
Sterilize by filtration through 0.45 µm membrane. Add 2.5 ml concentrate to 225 ml base.
Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.
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