-
Food
-
BAM Media M134: Selenite Cystine Broth
January 2001
Bacteriological Analytical Manual
M134
Selenite Cystine Broth
Medium 1 (modification of Leifson's formulation for selenite broth)
| Tryptone or polypeptone | 5 g |
| Lactose | 4 g |
| Sodium acid selenite (NaHSeO3) | 4 g |
| Na2HPO4 | 10 g |
| L-Cystine | 0.01 g |
| Distilled water | 1 liter |
Heat to boiling to dissolve. Dispense 10 ml portions into sterile 16 x 150 mm test tubes. Heat 10 min in flowing steam. DO NOT AUTOCLAVE. Final pH, 7.0 ± 0.2. The medium is not sterile. Use same day as prepared.
Medium 2 (North-Bartram modification)
| Polypeptone | 5 g |
| Lactose | 4 g |
| Sodium acid selenite (NaHSeO3) | 4 g |
| Na2HPO4 | 5.5 g |
| KH2PO4 | 4.5 g |
| L-Cystine | 0.01 g |
| Distilled water | 1 liter |
Heat with agitation to dissolve. Dispense 10 ml portions into sterile 16 x 150 mm test tubes. Heat 10 min in flowing steam. DO NOT AUTOCLAVE. Use same day as prepared.
Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.
-
-