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Food
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BAM Media M20a: Blood Agar Base
January 2001
Bacteriological Analytical Manual
M20a
Blood Agar Base
| Infusion from beef heart | 500 g |
| Tryptose | 10 g |
| NaCl | 5 g |
| Agar | 15 g |
| Sheep blood (sterile, defibrinated) |
50 ml |
| Distilled water | 1 liter |
Suspend ingredients except blood to dissolve. Autoclave 15 min at 121°C. Cool to 45-50°C. Add 50 ml blood (room temperature), and mix. Dispense into sterile petri dishes. Final pH, 6.8 ± 0.2.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.
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