-
Food
-
BAM Media M7: AKI Medium
January 2001
Bacteriological Analytical Manual
M7
AKI Medium
| Peptone | 15 g |
| Yeast extract | 4 g |
| NaCl | 5 g |
| Distilled water | 970 ml |
| NaHCO3, 10% aqueous, filter-sterilized |
30 ml |
On day of use, dissolve peptone, yeast extract, and NaCl in distilled water. Autoclave 15 min at 121°C. Cool. Add 30 ml freshly prepared, filter-sterilized NaHCO3, and mix. Dispense aseptically into screw-capped tubes (use 15 ml for 16 x 125 mm tubes). Final pH, 7.4 ± 0.2.
Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.
-
-