BAM Media M190: Vibrio vulnificus Agar (VVA)

May 2004

Bacteriological Analytical Manual

M190
Vibrio vulnificus Agar (VVA)

Solution 1

Adjust to pH 8.2. Boil to dissolve agar. Autoclave for 15 min at 121°C. Temper to 50°C.

*100X Dye stock solution

For consistent medium color, use dye solution rather than repeatedly weighing out dry dyes.

Solution 2

Dissolve cellobiose in distilled water by heating gently. Cool and filter sterilize.

Add Solution 2 to cooled Solution 1, mix, and dispense into petri dishes. Final color, light blue.

Medium may be stored 2 weeks at refrigeration temperatures.