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Food
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BAM Media M171: Urea Broth
January 2001; Updated February 2008
Bacteriological Analytical Manual
M171
Urea Broth
| Urea | 20 g |
| Yeast extract | 0.1 g |
| Na2HPO4 | 9.5 g |
| KH2PO4 | 9.1 g |
| Phenol red | 0.01 g |
| Distilled water | 1 liter |
Dissolve ingredients in distilled water. DO NOT HEAT. Sterilize by filtration through 0.45 µm membrane. Aseptically dispense 1.5-3.0 ml portions to 13 x 100 mm sterile test tubes. Final pH, 6.8 ± 0.2.
Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.
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