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Food
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BAM Media M166: Tryptose Blood Agar Base
January 2001
Bacteriological Analytical Manual
M166
Tryptose Blood Agar Base
| Tryptose | 10 g |
| Beef extract | 3 g |
| NaCl | 5 g |
| Agar | 15 g |
| Distilled water | 1 liter |
Suspend ingredients in distilled water, mix thoroughly, and heat with occasional agitation. Boil about 1 min. Fill 16 x 150 mm tubes 1/3 full and cap or plug to maintain aerobic conditions. Autoclave 15 min at 121°C. Before media solidify, incline tubes to obtain 4-5 cm slant and 2-3 cm butt.
Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.
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