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Food
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BAM Media M163: Tryptone Salt Agar
January 2001
Bacteriological Analytical Manual
M163
Tryptone Salt Agar
(T1N1 Agar and T1N2 Agar)
| Trypticase or tryptone | 10 g |
| NaCl | 10 g |
| Agar | 20 g |
| Distilled water | 1 liter |
Suspend ingredients and boil to dissolve agar. For slants, dispense into tubes. Autoclave 15 min at 121°C. Solidify tubes as slants. Cool medium for plates to 45-50°C and pour into sterile petri dishes.
For T1N2 agar, use 20 g NaCl rather than the 10 g specified for T1N1 agar.
Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.
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