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BAM R57: Peroxidase Substrate for Membrane ELISA

January 2001

Bacteriological Analytical Manual

R57
Peroxidase Substrate for Membrane ELISA

 

Solution A


4-Chloro-1-naphthol 60 mg
Methanol (cold) 20 ml

 

Dissolve 4-chloro-1-naphthol in cold methanol. Store at -20°C.


Solution B


Hydrogen peroxide, 30% 60 1
Tris-buffered saline (TBS) 100 ml

 

Add 30% H2O2 to TBS at room temperature.


Immediately before use, mix ice cold Solution A with room temperature Solution B.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

    
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