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Food
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BAM R49: North Aniline (Oil) - Methylene Blue Stain
January 2001
Bacteriological Analytical Manual
R49
North Aniline (Oil)-Methylene Blue Stain
| Aniline oil | 3 ml |
| Ethanol (95%) | 10 ml |
| HCl (concentrated) | 1.5 ml |
| Methylene blue (saturated solution) | 30 ml |
| Distilled water to make | 100 ml |
Mix aniline oil with ethanol. Slowly add HCl with constant agitation. Add saturated methylene blue solution, dilute to 100 ml with water, and filter.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.
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