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Food
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BAM R38: Kovacs' Reagent
January 2001
Bacteriological Analytical Manual
R38
Kovacs' Reagent
| p-Dimethylaminobenzaldehyde | 5 g |
| Amyl alcohol (normal only) | 75 ml |
| HCl (concentrated) | 25 ml |
Dissolve p-dimethylaminobenzaldehyde in normal amyl alcohol. Slowly add HCl. Store at 4°C. To test for indole, add 0.2-0.3 ml reagent to 5 ml of 24 h bacteria culture in tryptone broth. Dark red color in surface layer is positive test for indole.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998
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