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Food
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BAM R31: Glycerin-Salt Solution (Buffered)
January 2001
Bacteriological Analytical Manual
R31
Glycerin-Salt Solution (Buffered)
| Glycerin (reagent grade) | 100 ml |
| K2HPO4 (anhydrous) | 12.4 g |
| KH2PO4 (anhydrous) | 4 g |
| NaCl | 4.2 g |
| Distilled water | 900 ml |
Distilled NaCl and bring volume to 900 ml with water. Add glycerin and phosphates. Adjust pH to 7.2. Autoclave 15 min at 121°C. For double strength (20%) glycerin solution, use 200 ml glycerin and 800 ml distilled water.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.
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