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Food
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BAM R30: Giemsa Stain
January 2001
Bacteriological Analytical Manual
R30
Giemsa Stain
| Giemsa stain (Matheson Coleman & Bell, Norwood, OH 45212) |
1 g |
| Glycerol | 66 ml |
| Methanol (absolute) | 66 ml |
Distilled stain in glycerol by heating 1.5-2.0 h at 55-60°C. Add methanol. Store stain in tightly stoppered bottle at 22°C for at least 2 weeks. Dilute stock solution with distilled water (1+9) before use.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.
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