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BAM R19: Dulbecco's Phosphate-Buffered Saline (DPBS)

January 2001

Bacteriological Analytical Manual

R19
Dulbecco's Phosphate-Buffered Saline (DPBS)

 

NaCl 8.0 g
KCl 200 mg
Na2HPO4 1.15 g
KH2PO4 200 mg
CaCl2 100 mg
MgCl2ยท 6H2O 100 mg
Distilled Water 1 liter

 

Dissolve ingredients in water. Sterilize by filtration. Final pH, 7.2.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

    
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