BAM R11: Butterfield's Phosphate-Buffered Dilution Water

January 2001

Bacteriological Analytical Manual

R11
Butterfield's Phosphate-Buffered Dilution Water

Adjust pH to 7.2 with 1 N NaOH. Bring volume to 1 liter with distilled water. Sterilize 15 min at 121°C. Store in refrigerator.

Dilution blanks

Take 1.25 ml of above stock solution and bring volume to 1 liter with distilled water. Dispense into bottles to 90 or 99 ± 1 ml. Sterilize 15 min at 121°C.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.