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BAM Media M60: Heart Infusion (HI) Broth and Agar (HIA)

January 2001

Bacteriological Analytical Manual

Heart Infusion (HI) Broth and Agar (HIA)
(for Vibrio)

Beef heart, infusion from 500 g1 liter
Tryptose10 g
NaCl5 g


Dissolve ingredients and dispense into tubes.

For halophilic Vibrio spp. add an extra 15 g NaCl per liter.


Autoclave 15 min at 121°C. Final pH, 7.4 ± 0.2.

For heart infusion agar, add 15 g agar/liter and boil to dissolve before dispensing and sterilizing.

Commercially available from Difco.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.