Bacteriological Analytical Manual
|Beef heart, infusion from 500 g||1 liter|
Dissolve ingredients and dispense into tubes.
For halophilic Vibrio spp. add an extra 15 g NaCl per liter.
Autoclave 15 min at 121°C. Final pH, 7.4 ± 0.2.
For heart infusion agar, add 15 g agar/liter and boil to dissolve before dispensing and sterilizing.
Commercially available from Difco.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.