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U.S. Department of Health and Human Services

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RFE PagPlate Gel ppg01srr: Isoelectric Focusing Peak Report Data for Sebastes alutus; Pacific Ocean Perch; FDA Market Name: Perch, Ocean

Scientific Name: Sebastes alutus
Scientific Common Name: Pacific Ocean Perch
FDA Market Name: Perch, Ocean
Unique IEF Gel Code: 19980831gel02
Unique Web Code: ppg01Srr
Tissue Aliquot Code: sebaalut I94-001

Specific Lane Data

 

Lane a
pI Markers
Lane b
Co-Migrated
Lane c
Specimen
Lane d
pI Markers
Lane e
Specimen
Lane f
pI Markers

Schematic Gel Representation

 

RFE: IEF PagPlate Gel# ppg01srr: schematic gel image for Pacific Ocean Perch, Sebastes alutus, FDA Market Name: Perch, Ocean
Key to Gel Schematic:
  • The Gel Banding Pattern Display (image, left) was originally created in HTML using a gif image stacking technique based on migration position normalized data. The band intensity is based on original densitometry measurements.
  • MK=pI Marker Lanes, pI bands are in red
  • SP=Specimen Lanes, Specimen bands are in blue
  • CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
  • See the Lane illustrations on the Standardized Gel Configuration (image file)

  • Note:  In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment.  Red pI band is reported when overlapped peak position is equal to nominal pI Marker position.  Blue Specimen band is reported when overlapped peak position deviates slightly from nominal pI Marker position. Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.

Normalized Peak-Position Peak-Area for Copy-Paste Events


PeakCount: 121

DISPLAY GEL LANE: a 
DIGITAL REPORT GEL LANE: 16
LANE TYPE: pI Markers
ACTUAL GEL LANE: 17
PeakCount: 11

DISPLAY GEL LANE: b 
DIGITAL REPORT GEL LANE: 17
LANE TYPE: Specimen
ACTUAL GEL LANE: 18
PeakCount: 24

DISPLAY GEL LANE: c 
DIGITAL REPORT GEL LANE: 18
LANE TYPE: pI Markers
ACTUAL GEL LANE: 19
PeakCount: 11

DISPLAY GEL LANE: d 
DIGITAL REPORT GEL LANE: 19
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 20
PeakCount: 38

DISPLAY GEL LANE: e 
DIGITAL REPORT GEL LANE: 20
LANE TYPE: Specimen
ACTUAL GEL LANE: 21
PeakCount: 26

DISPLAY GEL LANE: f 
DIGITAL REPORT GEL LANE: 21
LANE TYPE: pI Markers
ACTUAL GEL LANE: 22
PeakCount: 11