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BAM Media M194: Tellurite Cefixime - Sorbitol MacConkey Agar (TC-SMAC)

August 2010

Bacteriological Analytical Manual

M194
Tellurite Cefixime – Sorbitol MacConkey Agar (TC-SMAC)

Prepare Sorbitol-MacConkey Agar (M-139) as directed. Once SMAC has been autoclaved and tempered, then add the 2 filter-sterilized additives below to obtain the final concentrations shown.

 

 

 

 

Potassium tellurite 2.5mg/L (250 µL of 1% solution)*
Cefixime 0.05 mg/L (1mL of 50 mg/L solution in 95% EtOH)

*Note: For 1% Potassium tellurite, store at 4° C for 1 month.

  For Cefixime, store at -20° C for 1 year.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998. Updated and revised: 29-DEC-2000; 20-AUG-2010.