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BAM Media M192a: Modified Buffered Peptone water with pyruvate (mBPWp) and Acriflavin-Cefsulodin-Vancomycin (ACV) Supplement

July 2009

Bacteriological Analytical Manual

M192a
Modified Buffered Peptone water with pyruvate (mBPWp)

Peptone 10.0 g
NaCl 5.0 g
Na2HPO4 3.6 g
KH2PO4 1.5 g
Casamino acids 5.0 g
Yeast extract 6.0 g
Lactose 10.0 g
Sodium Pyruvate 1.0g
Distilled water 1000 ml (Use 500 ml for 2X strength)

pH to 7.2 ± 0.2

Note: commercial BPW (20 g per L) can be substituted for the first four ingredients.

Sterilize by autoclaving.

Acriflavin-Cefsulodin-Vancomycin (ACV) Supplement for mBPWp

Supplement Conc. in mBPWp Conc. In stock Amt. of stock/ 225mL
Acriflavin 10mg/L 1.125 g/ 500 mL 1 mL
Cefsulodin 10mg/L 1.125 g/ 500 mL 1 mL
Vancomycin 8mg/L 0.90 g/ 500 mL 1 mL

Filter, sterilize. Add 1mL of each to 225 mL mBPWp for selective overnight enrichment.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998. Updated and revised: 29-DEC-2000.