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U.S. Department of Health and Human Services


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Bacteriological Analytical Manual (BAM) Media M34: Casamino Acids-Yeast Extract-Salts (CA-YE) Broth

January 2001


Casamino acids20 g
Yeast extract6 g
NaCl2.5 g
K2HPO48.71 g
Trace salts solution
(below), optional
1 ml
Distilled water1 liter


Adjust pH so that value after autoclaving is 8.5 ± 0.2. Autoclave 15 min at 121°C.


Trace salts solution (optional)

MgSO450 g
MnCl25 g
FeCl25 g
Distilled water1 liter


Suspend ingredients. Add enough 0.1 N H2SO4 to dissolve. Sterilize by filtration through 0.45 µm membrane. Add 1 ml to 1 liter base. Dispense 10 ml portions into 50 ml flasks.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.