Bacteriological Analytical Manual (BAM) Media M189: Cellobiose-Colistin (CC) Agar
|Beef extract||5 g|
|1000X Dye stock solution*||1 ml|
|Distilled water||900 ml|
Adjust to pH 7.6. Boil to dissolve agar. Cool to 48-55°C.
*1000X Dye stock solution
|Bromthymol blue||4.0 g|
|Cresol red||4.0 g|
|Ethanol, 95%||100 ml|
For consistent medium color, use dye solution rather than repeatedly weighing out dry dyes. Dissolve dyes in ethanol for 4% (w/v) stock solution. Using 1 ml of this solution per liter of mCPC agar gives 40 mg bromthymol blue and 40 mg cresol red per liter.
|Distilled water||100 ml|
Dissolve cellobiose in distilled water by heating gently. Cool. Add antibiotics and filter sterilize.
Add Solution 2 to cooled Solution 1, mix, and dispense into petri dishes. Final color, dark green to green-brown.
NOTE: This medium, like TCBS, is very inhibitory and does not require autoclaving. Medium may be stored 2 weeks at refrigeration temperatures.
|Storage:||limit: 2 wks||Temperature: 4°C|