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BAM Media M37: Cetrimide Agar

January 2001

Bacteriological Analytical Manual

Cetrimide Agar

Pancreatic digest of gelatin20.0 g
Magnesium chloride1.4 g
Potassium sulfate10.0 g
Agar13.6 g
(cetyl trimethyl ammonium bromide)
0.3 g


Suspend 45.3 g of ingredients or commercial powder (PseudoselTM, BBL; Becton-Dickinson) in 1 liter of purified water. Add 10 ml glycerol. Mix well. Heat to boiling, agitating frequently; maintain boiling for 1 min to dissolve powder.

Autoclave at 118°C for 15 min.


Final pH, 7.2 ± 0.2. Bacto cetrimide agar base plus glycerol (Difco) is a similar medium.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.