Bacteriological Analytical Manual
|Pancreatic digest of gelatin||20.0 g|
|Magnesium chloride||1.4 g|
|Potassium sulfate||10.0 g|
(cetyl trimethyl ammonium bromide)
Suspend 45.3 g of ingredients or commercial powder (PseudoselTM, BBL; Becton-Dickinson) in 1 liter of purified water. Add 10 ml glycerol. Mix well. Heat to boiling, agitating frequently; maintain boiling for 1 min to dissolve powder.
Autoclave at 118°C for 15 min.
Final pH, 7.2 ± 0.2. Bacto cetrimide agar base plus glycerol (Difco) is a similar medium.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.