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U.S. Department of Health and Human Services

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BAM Media M38: Chopped Liver Broth

January 2001

Bacteriological Analytical Manual

M38
Chopped Liver Broth

Fresh beef liver500 g
Peptone10 g
K2HPO41 g
Soluble starch1 g
Distilled water1 liter

 

Grind liver into the water. Heat to boiling and simmer 1 h. Cool, adjust pH to 7.0, and boil 10 min. Filter through cheesecloth and press out excess liquid. Add other ingredients and adjust pH to 7.0. Add water to make 1 liter. Filter through coarse filter paper.


 

Store broth and meat separately in freezer. To 18 x 150 or 20 x 150 mm test tubes, add chopped liver to depth of 1.2-2.5 cm and 10-12 ml of broth. Autoclave 15 min at 121°C.

 


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.