BAM Media M38: Chopped Liver Broth

January 2001

Bacteriological Analytical Manual

Fresh beef liver 500 g
Peptone 10 g
K2HPO4 1 g
Soluble starch 1 g
Distilled water 1 liter

Grind liver into the water. Heat to boiling and simmer 1 h. Cool, adjust pH to 7.0, and boil 10 min. Filter through cheesecloth and press out excess liquid. Add other ingredients and adjust pH to 7.0. Add water to make 1 liter. Filter through coarse filter paper.

Store broth and meat separately in freezer. To 18 × 150 or 20 × 150 mm test tubes, add chopped liver to depth of 1.2-2.5 cm and 10-12 ml of broth. Autoclave 15 min at 121°C.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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