Bacteriological Analytical Manual
|Malt extract, powdered||20.0 g|
|Distilled water||1.0 liter|
Mix ingredients, steam to dissolve agar and sterilize for 15 min at 121°C. Temper medium to 45°C and pour plates under aseptic conditions.
To prepare slants dispense 5-6 ml of steamed medium (before autoclaving) into each of several 16 × 125 mm screw-cap tubes, loosely cap tubes and sterilize as above. After autoclaving lay tubes in a slanting position and let them cool.
This medium is recommended as a general maintenance medium.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.