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BAM Media M92: Malonate Broth

January 2001

Bacteriological Analytical Manual

Malonate Broth

Yeast extract1 g
(NH4)2SO42 g
K2HPO40.6 g
KH2PO40.4 g
NaCl2 g
Sodium malonate3 g
Glucose0.25 g
Bromthymol blue0.025 g
Distilled water1 liter


Dissolve by heating, if necessary. Dispense 3 ml portions into 13 x 100 mm test tubes. Autoclave 15 min at 121°C. Final pH, 6.7 ± 0.2.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.