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BAM Media M90: Lysozyme Broth

January 2001

Bacteriological Analytical Manual

M90
Lysozyme Broth


 

Base

Prepare nutrient broth as recommended. Dispense 99 ml portions to 170 ml bottles. Autoclave 15 min at 121°C. Cool to room temperature before use.


 

Lysozyme solution

Dissolve 0.1 g lysozyme in 65 ml sterile 0.0l N HCl. Heat to boiling for 20 min. Dilute to 100 ml with sterile 0.0l N HCl. Alternatively, dissolve 0.1 g lysozyme in 100 ml distilled water. Sterilize by filtration through 0.45 µm membrane. Test for sterility before use. Add 1 ml lysozyme solution to 99 ml nutrient broth. Mix and dispense 2.5 ml portions to sterile 13 x 100 mm tubes.


 


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.