Bacteriological Analytical Manual
|Gelysate or peptone||5 g|
|Yeast extract||3 g|
|L-Lysine hydrochloride||10 g|
|Ferric ammonium citrate||0.5 g|
|Sodium thiosulfate (anhydrous)||0.04 g|
|Bromcresol purple||0.02 g|
|Distilled water||1 liter|
Heat to dissolve ingredients. Dispense 4 ml portions into 13 × 100 mm screw-cap tubes. Autoclave 12 min at 121°C. Let solidify in slanted position to form 4 cm butts and 2.5 cm slants. Final pH, 6.7 ± 0.2.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.