BAM Media M87: Lysine Decarboxylase Broth (Falkow) (for Salmonella)

January 2001

Bacteriological Analytical Manual

Gelysate or peptone 5 g
Yeast extract 3 g
Glucose 1 g
L-Lysine 5 g
Bromcresol purple 0.02 g
Distilled water 1 liter

Heat until dissolved. Dispense 5 ml portions into 16 × 125 mm screw-cap tubes. Autoclave loosely capped tubes 15 min at 121°C. Screw the caps on tightly for storage and after inoculation. Final pH, 6.8 ± 0.2.

For halophilic Vibrio spp., add NaCl to a final concentration of 2-3%.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

Page Last Updated: 08/05/2015
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