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BAM Media M87: Lysine Decarboxylase Broth

January 2001

Bacteriological Analytical Manual

M87
Lysine Decarboxylase Broth
(Falkow)
(for Salmonella)

Gelysate or peptone5 g
Yeast extract3 g
Glucose1 g
L-Lysine5 g
Bromcresol purple0.02 g
Distilled water1 liter

 

Heat until dissolved. Dispense 5 ml portions into 16 x 125 mm screw-cap tubes. Autoclave loosely capped tubes 15 min at 121°C. Screw the caps on tightly for storage and after inoculation. Final pH, 6.8 ± 0.2.

 

For halophilic Vibrio spp., add NaCl to a final concentration of 2-3%.

 


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.