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BAM Media M86: Lysine Arginine Iron Agar (LAIA)

January 2001

Bacteriological Analytical Manual

Lysine Arginine Iron Agar (LAIA)

Peptone5 g
Yeast extract3 g
Glucose1 g
L-Lysine10 g
L-Arginine10 g
Ferric ammonium citrate0.5 g
Sodium thiosulfate0.04 g
Bromcresol purple0.02 g
Agar15 g


Adjust pH to 6.8. Heat to boiling and dispense 5 ml into each 13 x 100 mm screw-cap culture tube. Autoclave at 121°C for 12 min. Cool tubes in slanted position. (This medium may also be prepared by supplementing Difco lysine iron agar (LIA) with 10 g L-arginine per liter.)


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.