Bacteriological Analytical Manual
|Yeast extract||3 g|
|Ferric ammonium citrate||0.5 g|
|Sodium thiosulfate||0.04 g|
|Bromcresol purple||0.02 g|
Adjust pH to 6.8. Heat to boiling and dispense 5 ml into each 13 × 100 mm screw-cap culture tube. Autoclave at 121°C for 12 min. Cool tubes in slanted position. (This medium may also be prepared by supplementing Difco lysine iron agar (LIA) with 10 g L-arginine per liter.)
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.