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BAM Media M85: Long-term Preservation Medium

January 2001

Bacteriological Analytical Manual

Long-term Preservation Medium

Yeast extract, 0.3%3 g
Peptone10 g
NaCl30 g
Agar3 g
Distilled water1 liter


Heat to dissolve ingredients. Dispense 4 ml portions to 13 x 100 mm screw-cap tubes. Autoclave 15 min at 121°C. Cool and tighten caps for storage. No pH adjustment is necessary.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.