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BAM Media M81: Lithium Chloride-Phenylethanol-Moxalactam (LPM) Medium

January 2001

Bacteriological Analytical Manual

M81
Lithium Chloride-Phenylethanol-Moxalactam (LPM) Medium

Phenylethanol agar (Difco)35.5 g
Glycine anhydride
( NOTE: not glycine)
10 g
Lithium chloride5 g
Moxalactam stock solution,
1% in phosphate buffer, pH 6.0
2 ml
Distilled water1 liter

 

Sterilize medium (without moxalactam) at 121°C for 15 min. Cool to 48-50°C and add filter-sterilized moxalactam solution.

 

Moxalactam stock solution

Consists of 1 g moxalactam salt (ammonium or sodium) in 100 ml 0.1 M potassium phosphate buffer, pH 6.0. Store filter-sterilized stock solution frozen in 2 ml aliquots.

Moxalactam (Eli Lilly Co.) is retailed by Sigma Chemical Co. The LPM medium is most effective in the Henry illumination system when poured thin, i.e., 12-15 ml per standard petri dish. To avoid drying of thin agar, refrigerate and use plates rapidly. LPM basal medium is commercially available as a powder.

 


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.