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BAM Media M79: Letheen Broth (Modified)

January 2001

Bacteriological Analytical Manual

Letheen Broth (Modified)

Letheen broth25.7 g
Trypticase peptone5 g
Thiotone peptone10 g
Yeast extract2 g
Sodium bisulfite0.1 g
Distilled water1 liter


Dispense 90 ml into screw-cap bottle. Autoclave 15 min at 121°C. Final pH, 7.2 ± 0.2.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.
Revisions: 2001-AUG-20 Formulation corrected, letheen broth amount corrected from 26.7 to 25.7.