BAM Media M78: Letheen Agar (Modified)

January 2001

Bacteriological Analytical Manual

Letheen agar (Difco or BBL) 32 g
Trypticase peptone 5 g
Thiotone peptone 10 g
Yeast extract 2 g
NaCl 5 g
Sodium bisulfite 0.1 g
Agar 5 g
Distilled water 1 liter

Heat with agitation to dissolve agar. Autoclave 15 min at 121°C. Aseptically dispense 20 ml into 15 × 100 mm petri dishes. Final pH, 7.2 ± 0.2.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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