Bacteriological Analytical Manual
|Letheen agar (Difco or BBL)||32 g|
|Trypticase peptone||5 g|
|Thiotone peptone||10 g|
|Yeast extract||2 g|
|Sodium bisulfite||0.1 g|
|Distilled water||1 liter|
Heat with agitation to dissolve agar. Autoclave 15 min at 121°C. Aseptically dispense 20 ml into 15 × 100 mm petri dishes. Final pH, 7.2 ± 0.2.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.