• Decrease font size
  • Return font size to normal
  • Increase font size
U.S. Department of Health and Human Services

Food

  • Print
  • Share
  • E-mail

BAM Media M78: Letheen Agar (Modified)

January 2001

Bacteriological Analytical Manual

M78
Letheen Agar (Modified)

Letheen agar (Difco or BBL)32 g
Trypticase peptone5 g
Thiotone peptone10 g
Yeast extract2 g
NaCl5 g
Sodium bisulfite0.1 g
Agar5 g
Distilled water1 liter

 

Heat with agitation to dissolve agar. Autoclave 15 min at 121°C. Aseptically dispense 20 ml into 15 x 100 mm petri dishes. Final pH, 7.2 ± 0.2.

 


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.