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BAM Media M78: Letheen Agar (Modified)

January 2001

Bacteriological Analytical Manual

Letheen Agar (Modified)

Letheen agar (Difco or BBL)32 g
Trypticase peptone5 g
Thiotone peptone10 g
Yeast extract2 g
NaCl5 g
Sodium bisulfite0.1 g
Agar5 g
Distilled water1 liter


Heat with agitation to dissolve agar. Autoclave 15 min at 121°C. Aseptically dispense 20 ml into 15 x 100 mm petri dishes. Final pH, 7.2 ± 0.2.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.