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BAM Media M77: Lauryl Tryptose MUG (LST-MUG) Broth

January 2001

Bacteriological Analytical Manual

Lauryl Tryptose MUG (LST-MUG) Broth

Tryptose or trypticase20 g
Lactose5 g
K2HPO42.75 g
KH2PO42.75 g
NaCl5 g
Sodium lauryl sulfate0.1 g
β-D-glucuronide (MUG)*
50 mg
Distilled water1 liter


Prepare lauryl tryptose broth and add MUG. Dissolve with gentle heat if necessary. Dispense 10 ml portions into 20 x 150 mm test tubes containing inverted 10 x 75 mm fermentation tubes. Autoclave 15 min at 121°C. Final pH, 6.8 ± 0.2.


*MUG may be obtained from several sources, e.g., HACH Co., Loveland, CO.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.