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U.S. Department of Health and Human Services


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BAM Media M74: Lactose Broth

January 2001

Bacteriological Analytical Manual

Lactose Broth

Beef extract3 g
Peptone5 g
Lactose5 g
Distilled water1 liter


Dispense 225 ml portions into 500 ml Erlenmeyer flasks. After autoclaving 15 min at 121°C and just before use, aseptically adjust volume to 225 ml. Final pH, 6.9 ± 0.2.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.