BAM Media M73: L-15 Medium (Modified) Leibovitz

January 2001

Bacteriological Analytical Manual

L-15 Medium (Modified) Leibovitz

D-Galactose90 mgL-Tyrosine300 mg
Phenol red, Na10 mgDL-Valine200 mg
Sodium pyruvate550 mgD-Calcium pantothenate1 mg
DL--Alanine450 mgCholine chloride1 mg
L-Arginine (free base)500 mgFolic acid1 mg
L-Asparagine·H2O250 mgi-Inositol2 mg
L-Cysteine (free base)120 mgNicotinamide1 mg
L-Glutamine300 mgPyridoxine HCl1 mg
Glycine200 mgRiboflavin-5-phosphate, Na0.1 mg
L-Histidine (free base)250 mgThiamin
1.0 mg
L-Isoleucine250 mgCaCl2 (anhydrous)140 mg
L-Leucine HC1125 mgKC1400 mg
DL-Methionine150 mgKH2PO460 mg
L-Phenylalanine250 mgMgCl2 (anhydrous)93.68 mg
L-Serine200 mgNaCl8000 mg
DL-Threonine600 mgNa2HPO4 (anhydrous)190 mg
L-Tryptophan20 mgDistilled water1 liter

Filter through 0.20 µm membrane and dispense into 2 liter Erlenmeyer flask. Final pH, 7.5. Cap and store at 5°C.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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