Food

BAM Media M73: L-15 Medium (Modified) Leibovitz

January 2001

Bacteriological Analytical Manual

D-Galactose 90 mg L-Tyrosine 300 mg
Phenol red, Na 10 mg DL-Valine 200 mg
Sodium pyruvate 550 mg D-Calcium pantothenate 1 mg
DL--Alanine 450 mg Choline chloride 1 mg
L-Arginine (free base) 500 mg Folic acid 1 mg
L-Asparagine·H2O 250 mg i-Inositol 2 mg
L-Cysteine (free base) 120 mg Nicotinamide 1 mg
L-Glutamine 300 mg Pyridoxine HCl 1 mg
Glycine 200 mg Riboflavin-5-phosphate, Na 0.1 mg
L-Histidine (free base) 250 mg Thiamin
monophosphate·2H2O
1.0 mg
L-Isoleucine 250 mg CaCl2 (anhydrous) 140 mg
L-Leucine HC1 125 mg KC1 400 mg
DL-Methionine 150 mg KH2PO4 60 mg
L-Phenylalanine 250 mg MgCl2 (anhydrous) 93.68 mg
L-Serine 200 mg NaCl 8000 mg
DL-Threonine 600 mg Na2HPO4 (anhydrous) 190 mg
L-Tryptophan 20 mg Distilled water 1 liter

Filter through 0.20 µm membrane and dispense into 2 liter Erlenmeyer flask. Final pH, 7.5. Cap and store at 5°C.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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