• Decrease font size
  • Return font size to normal
  • Increase font size
U.S. Department of Health and Human Services

Food

  • Print
  • Share
  • E-mail

BAM Media M72: Koser's Citrate Broth

January 2001

Bacteriological Analytical Manual

M72
Koser's Citrate Broth

NaNH4HPO4·4H2O1.5 g
KH2PO4 (monobasic)1 g
MgSO4·7H2O0.2 g
Sodium citrate·2H2O3 g
Distilled water1 liter

 

Dispense into screw-cap tubes as desired. Autoclave 15 min at 121°C. Final pH, 6.7 ± 0.2. This formulation is listed in Official Methods of Analysis of AOAC International, and Standard Methods for the Examination of Wastewater of the APHA. It differs from the composition of commercially available dehydrated medium. The latter is satisfactory.

 


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.