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BAM Media M71: Kligler Iron Agar

January 2001

Bacteriological Analytical Manual

Kligler Iron Agar

Polypeptone peptone20 g
Lactose20 g
Dextrose1 g
NaCl5 g
Ferric ammonium citrate0.5 g
Sodium thiosulfate0.5 g
Agar15 g
Phenol red0.025 g
Distilled water1 liter


Heat with agitation to dissolve. Dispense into 13 x 100 mm screw-cap tubes and autoclave 15 min at 121°C. Cool and slant to form deep butts. Final pH, 7.4 ± 0.2.

Commercially available from Difco, BBL, and Oxoid.


For use with halophilic Vibrio spp., add NaCl to a final concentration of 2-3%.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.