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BAM Media M70: King's O/F Basal Medium

January 2001

Bacteriological Analytical Manual

King's O/F Basal Medium



Trypticase (or casitone)2 g
Phenol red, 1.5% solution2 ml
Agar3 g
Distilled water1 liter


Heat with agitation to dissolve agar. Adjust pH to 7.3 ± 0.2. Dispense 100 ml portions to flasks. Autoclave 15 min at 121°C. Cool to 50°C.


Carbohydrates, 10%.


Dissolve 10 g quantities of carbohydrates in 100 ml distilled water. Sterilize by filtration through 0.22 µm membrane. Add 10 ml concentrate to 90 ml melted base and mix. Aseptically dispense 3 ml portions to sterile 13 x 100 mm tubes.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.