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BAM Media M69: King's B Medium

January 2001

Bacteriological Analytical Manual

King's B Medium

Proteose peptone No. 320 g
Glycerol, C.P.10 ml
K2HPO41.5 g
MgSO41.5 g
Agar15 g
Distilled water1 liter


Add all ingredients except MgSO4. Heat with agitation to dissolve agar. Adjust pH to 7.2 ± 0.2. Add MgSO4 slowly and mix. Dispense 4 ml portions to 13 x 100 tubes. Autoclave 15 min at 121°C.


Incline tubes to give half butt and half slant.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.