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U.S. Department of Health and Human Services


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Bacteriological Analytical Manual (BAM) Media M68: Iron Milk Medium (Modified)

January 2001

Fresh whole milk1 liter
Ferrous sulfate.7H2O1 g
Distilled water50 ml


Dissolve ferrous sulfate in 50 ml distilled water. Add slowly to 1 liter milk and mix with magnetic stirrer. Dispense 11 ml medium into 16 x 150 mm culture tubes. Autoclave 12 min at 118°C.


Prepare fresh medium before use.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.