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U.S. Department of Health and Human Services


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BAM Media M66: Indole Nitrite Medium

January 2001

Bacteriological Analytical Manual

Indole Nitrite Medium
(Tryptic Nitrate)

Trypticase20 g
Na2HPO42 g
Dextrose1 g
KNO31 g
Agar1 g
Distilled water1 liter


Heat with agitation to dissolve ingredients. Mix and dispense 11 ml portions into 16 x 150 mm tubes. Autoclave 15 min at 118°C. Final pH, 7.2 ± 0.2.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.