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BAM Media M63: Hugh-Leifson Glucose Broth (HLGB)

January 2001

Bacteriological Analytical Manual

Hugh-Leifson Glucose Broth (HLGB)

Peptone2 g
Yeast extract0.5 g
NaCl30 g
Dextrose10 g
Bromcresol purple0.015 g
Agar3 g
Distilled water1 liter



Heat with agitation to dissolve agar. Adjust pH to 7.4 ± 0.2. Autoclave 15 min at 121°C.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.