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Bacteriological Analytical Manual (BAM) Media M62: HC (Hemorrhagic colitis E. coli strains) Agar

January 2001

Tryptone20.0 g
Bile salts No. 31.12 g
NaCl5.0 g
Sorbitol20.0 g
MUG reagent0.1 g
Bromcresol purple0.015 g
Agar15.0 g
Distilled water (deionized)1 liter

 

Dissolve ingredients in distilled water by heating with stirring. Autoclave 15 min at 121°C. Final pH, 7.2 ± 0.2.

NOTE: The MUG reagent is not essential for the enzyme-labeled monoclonal antibody procedure.

For DNA probe: If colonies are not to be isolated by growth and metabolic characteristics, MUG reagent may be omitted. Plates may be kept 3-4 weeks if wrapped and stored at 4°C. Colonies do not attach well on filters if plates are too dry.

 

If HC agar is to be utilized fully, see Chapter 24, ref. 105.

MUG reagent may be purchased from HACH Company, P.O. Box 389, Loveland, CO.

 

Storage:3-4 wk at 4°C if plates wrapped.

 


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.