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U.S. Department of Health and Human Services


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Bacteriological Analytical Manual (BAM) Media M61: Hektoen Enteric (HE) Agar

January 2001; updated August 2010 

StorageMay be stored up to 30 days under refrigeration (4 ± 2°C).


Peptone12 gNaCl5 g
Yeast extract3 gSodium thiosulfate5 g
Bile salts No. 39 gFerric ammonium citrate1.5 g
Lactose12 gBromthymol blue0.065 g
Sucrose12 gAcid fuchsin0.1 g
Salicin2 gAgar14.0 g
Distilled water1 liter


Heat to boiling with frequent agitation to dissolve. Boil no longer than 1 min. Do not overheat. Cool in water bath. Pour 20 ml portions into sterile 15 x 100 mm petri dishes. Let dry 2 h with lids partially removed. Final pH, 7.5 ± 0.2.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.