Bacteriological Analytical Manual (BAM) Media M61: Hektoen Enteric (HE) Agar
January 2001; updated August 2010
|Storage||May be stored up to 30 days under refrigeration (4 ± 2°C).|
|Peptone||12 g||NaCl||5 g|
|Yeast extract||3 g||Sodium thiosulfate||5 g|
|Bile salts No. 3||9 g||Ferric ammonium citrate||1.5 g|
|Lactose||12 g||Bromthymol blue||0.065 g|
|Sucrose||12 g||Acid fuchsin||0.1 g|
|Salicin||2 g||Agar||14.0 g|
|Distilled water||1 liter|
Heat to boiling with frequent agitation to dissolve. Boil no longer than 1 min. Do not overheat. Cool in water bath. Pour 20 ml portions into sterile 15 x 100 mm petri dishes. Let dry 2 h with lids partially removed. Final pH, 7.5 ± 0.2.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.