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U.S. Department of Health and Human Services


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Bacteriological Analytical Manual (BAM) Media M59: Heart infusion agar

January 2001

Heart infusion agar (HIA)


Beef heart infusion (Difco)500 g
Tryptose10 g
NaCl5 g
Agar15 g
Distilled water1 liter




Blood Agar Base No. 2 (Difco)1 liter
Proteose peptone15 g
Liver digest2.5 g
Yeast extract5 g
NaCl5 g
Agar12 g


Autoclave either base at 121°C for 15 min. If preparing blood agar, reduce water to 950 ml. Add 50 ml defibrinated (whole or lysed) horse blood and FBP (4 ml to agar + blood) after autoclaving and cooling to 48°C. Final pH, 7.4 ± 0.2.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.