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BAM Media M58: Ham's F-10 Medium

January 2001

Bacteriological Analytical Manual

Ham's F-10 Medium

(commercial preparation is preferred)

L-Alanine8.91 mgSodium pyruvate110 mg
L-Arginine Hcl211.00 mgThymidine0.727 mg
L-Asparagine-H2O15.00 mgBiotin0.024 mg
L-Aspartic acid13.30 mgCholine chloride0.698 mg
L-Cysteine·2HCl35.12 mgFolic acid1.320 mg
L-Glutamine146.20 mgIsoinositol0.541 mg
L-Glutamic acid14.70 mgNiacinamide0.615 mg
Glycine7.51 mgD-Calcium pantothenate0.715 mg
L-Histidine HCl·H2O21.00 mgPyridoxine HCl0.206 mg
L-Isoleucine2.60 mgRiboflavin0.376 mg
L-Leucine13.10 mgThiamine HCl1.010 mg
L-Lysine HCl29.30 mgVitamin B121.360 mg
L-Methionine4.48 mgCaCl2·2H2O44.10 mg
D-Phenylalanine4.96 mgCuSO4·5H2O0.0025 mg
L-Proline11.50 mgFeSO4·7H2O0.83 mg
L-Serine10.50 mgKCl285.00 mg
L-Threonine3.57 mgKH2PO483.00 mg
L-Tryptophan0.60 mgMgSO4·7H2O152.80 mg
L-Tyrosine1.81 mgNaCl7400.00 mg
L-Valine3.50 mgNaHCO31200.00 mg
Glucose1100.0 mgNaH2PO4·H2O290.0 mg
Hypoxanthine4.08 mgZnSO4·7H2O0.028 mg
Lipoic acid0.2 mgPhenol red1.2 mg
Distilled water1 liter


Dissolve ingredients in water. Sterilize by filtration. Final pH, 7.0 ± 0.2. Check sterility before use.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.