Bacteriological Analytical Manual
|Yeast extract||1 g|
|Distilled water||1 liter|
Suspend ingredients with constant stirring to prevent scorching gelatin, and boil to dissolve gelatin and agar. Adjust to pH 7.2 ± 0.2. Autoclave 15 min at 121°C. Cool to 45-50°C. Pour plates.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.